Bioengineered Nanofiber Scaffold Loaded with Resveratrol Improves Cardiac Function Following Myocardial Infarction After Four Weeks
*Jacob D. Campbell, *Vaithinathan Selvaraju, *Rajesh Lakshmanan, *Mahesh Thirunavukkarasu, David W. McFadden, *Nilanjana Maulik
University of Connecticut Health Center, Farmington, CT
Objective: To determine if an engineered nanofiber scaffold loaded (poly-caprolactone, or PCL) with the pro-angiogenic molecule resveratrol (PCL+R) has an impact on cardiac function following topical implantation after myocardial infarction (MI).
Design: Preclinical murine MI model
Setting: Basic Science Surgical Laboratory (UCHC)
Patients: ICR-CD1 mice aged 8-12 weeks were used for the study
Interventions: Mice underwent LAD occlusion or sham surgery. If LAD occlusion occurred, mice were further divided into MI alone, MI with added PCL, or MI with PCL+R.
Main Outcome Measure: The main outcome measure was left ventricular function, as measured by ejection fraction (EF), fractional shortening (FS), stroke volume (SV), and cardiac output (CO) through echocardiogram. Significance is set at p<0.05.
Results: The MI+PCL-R group showed improvement in EF (45.28 ± 3.7% vs 32.67 ± 2.97%), FS (22.85 ± 2.15% vs 15.59 ± 1.54), SV (50.9 ± 3.94µL vs 33.86 ± 3.04 µL), and CO (22.302 ±2.46 mL/min vs 14.09 ± 1.32 mL/min) compared to the MI+PCL. With respect to the MI, EF (30.85 ± 3.22%) and FS (14.80 ± 1.68%) were reduced compared to MI+PCL+R. SV and CO also improved compared to MI, but there was no statistical significance. We also observed increased formation of functional capillaries on the resveratrol treated scaffolds after MI compared to MI+PCL.
Conclusion: In this study resveratrol used as a slow release topical therapy, with the help of a semi-synthetic scaffold support/delivery system, following MI. The functional improvement in cardiac function was observed probably due to the reestablishment of blood vessels and restoration of collagen extracellular matrix in cardiac ischemic scar tissue aided through the resveratrol.
