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The Effect of Geraniol on a Murine Model of Colitis
*Sara A Morrison, *Sulaiman Hamarneh, *Mohammad Gharedaghi, *Tanit Phupitakphol, *Haizhong Huo, *Weifeng Zhang, *Dong Hu, *Wei Lu, *Konstantinos Economopoulos, *Sarah Gul, Richard A. Hodin
Massachusetts General Hospital, Boston, MA
Objective:
The objective of this study was to test the effect of Geraniol, an essential plant oil known for its anti-inflammatory properties, on the severity of colitis in a murine model.
Design:
Experimental groups consisted of a control group who received a routine diet of rodent chow and clean ionized water, a group who received drinking water with 3% DSS, and a treatment group which received drinking water with 3% DSS and Geraniol added. Experimental groups received 3% DSS for 7 days, and were sacrificed on the 8th day.
Setting:
Mice were housed in the CCM facilities at Massachusetts General Hospital.
Patients:
7 week old female wild type C57BL/6 mice were used in this study.
Interventions:
As Above.
Main Outcome Measures:
Animals were evaluated for weight daily. At the time of sacrifice, they underwent scoring based on their disease acuity index. Histologic slides of the colon were also scored. Additionally, the RNA of colonic samples was analyzed via qPCR for various inflammatory markers.
Results:
Treatment with Geraniol resulted in significantly decreased disease acuity scores (DSS vs. Geraniol: 8.0 vs. 2.4, p=0.019), as well as decreased weight loss (average weight loss DSS vs. Geraniol: 2.4g vs. 0.28g, p=0.007). The mice treated with Geraniol also exhibited lower histology scores (DSS vs. Geraniol: 3.5 vs. 2.54, p=0.05). Treatment with Geraniol resulted in marked reductions in RNA levels of a number of inflammatory markers (DSS vs. Geraniol: IL-1b: 46.4 vs. 20.4, p=0.14, IL-6: 132.1 vs. 2.58, p=0.08, TLR4: 82.3 vs. 2.97, p=0.07, CD-14: 76.04 vs. 5.31, p=0.09, iNOS: 0.45 vs. 0.1, p=0.12).
Conclusions:
Geraniol is effective in decreasing the severity of DSS colitis in a murine model.
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