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Continuous Cold Perfusion vs. Static Cold Preservation Comparison for Intestinal Graft Preservation in Rats
*Armando Salim Munoz-Abraham, *Abedalrazaq Alkukhun, *Sami Judeeba, *Roger Patrón-Lozano, *Tariq Alfadda, Manuel I Rodriguez-Davalos, John P. Geibel
Yale University School of Medicine, New Haven, CT
Objective:
Assess the optimal preservation techniques for intestinal grafts by comparing static cold preservation against continuous cold perfusion in a rats.
Design:
Comparison between static cold preservation (Control Group) with University of Wisconsin (UW) Preservation Solution versus Continuous Cold Perfusion with UW(UW Perfusion Group) or a Modified Preservation Solution (MPS) containing UW with L-Arginine (MPS Perfusion Group) with rat intestinal grafts.
Setting:
Laboratory of Surgical Research.
Patients:
Rats
Interventions:
30 cms. of Male rats’s small intestine were procured. Two intestinal loops (10 cm each) were then connected to two custom perfusion chambers of an intestinal perfusion device. One chamber received a constant flow of UW solution and the other MPS solution and both submerged in a bath of deionized water at 4°C. A third intestinal loop was placed in cold static preservation with UW solution. Intestinal tissue samples were taken at T0, T1 (1 hr), T3 (3 hrs.), and T6 (6 hrs.), fixed in Formalin at 10%, and sent to pathology for processing, analysis, and grading according to Park/Chiu Scoring System for Grading Intestinal Ischemia.
Main Outcome Measures:
Intestinal Mucosa preservation.
Results:
Both the UW and the MPS Perfusion groups better preserved the intestine in comparison to the static cold preservation after T6.
Conclusions:
Continuous cold perfusion can better preserve intestinal grafts when compared to static cold preservation. These early results might open the door for new techniques for intestine preservation for transplantation that can eventually be applied in the clinical setting.
| Tissue Sample | Park/Chiu Classification | Description | | Sample T0 | 0 | Normal Mucosa | | Control Static Cold T1 | 0 | Normal Mucosa | | UW Perfusion T1 | 0 | Normal Mucosa | | MPS Perfusion T1 | 0 | Normal Mucosa | | Control Static Cold T3 | 1 | Subepithelial bleb at tip of the villus | | UW Perfusion T3 | 0 | Normal Mucosa | | MPS Perfusion T3 | 0 | Normal Mucosa | | Control Static Cold T6 | 2 | More extended subepithelial space | | UW Perfusion T6 | 0 | Normal Mucosa | | MPS Perfusion T6 | 0 | Normal Mucosa |
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